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Genetic element
(GENE)
published: 25 Oct 2016
last updated: 02 Aug 2022
2-deoxyglucose-6-phosphate phosphatase 1
EN
-
DOG1EN
CS-dog1-YEASX
EN
Protein coding sequence
No
-
BCH-ORGA-SCBD-45724-5 Organism Saccharomyces cerevisiae (Yeast, YEASX)Fungi
EN
2-desoxyglucose-6-phosphate phosphatase
EN
In plant cells a glucose analog, 2-deoxyglucose (2-DOG), is phosphorylated by hexokinase to form 2-DOG-6-phosphate, which competes with glucose-6-phosphate causing cell death through the inhibition of glycolysis, protein synthesis, cell wall polysaccharide synthesis and also protein glycosylation.
DOGR1 gene, which has been isolated from Saccharomyces cerevisiae strain S288C, encodes 2-deoxyglucose-6-phosphate. The enzyme has the ability to convert toxic 2-DOG-6-phosphate to non-toxic products through dephosphorylation.
Therefore, transformed plant cells which carry the DOGR1 gene can be selected on medium containing 2-DOG.
EN
DOGR1 gene, which has been isolated from Saccharomyces cerevisiae strain S288C, encodes 2-deoxyglucose-6-phosphate. The enzyme has the ability to convert toxic 2-DOG-6-phosphate to non-toxic products through dephosphorylation.
Therefore, transformed plant cells which carry the DOGR1 gene can be selected on medium containing 2-DOG.
EN
- UniProtKB - P38774 (DOG1_YEAST) [ English ]
- 2-Deoxyglucose resistance: a novel selection marker for plant transformation. [ English ]
- Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens [ English ]
- Saccharomyces Genome Database - 2-deoxyglucose-6-phosphatase activity [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Living Modified Organism | Introduced or modified genetic element(s) | 1 | |
| Living Modified Organism | Genetic elements construct | 1 | |
